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4R023-L3-P01-CGATGT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L3-P01-CGATGT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L3-P01-CGATGT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51077047
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT194150.014.3104361
TCGAATG106950.013.1862632
CTCGTTT188150.012.9240531
GTCGGTT97150.012.1050481
CTCCGAT162450.012.0845341
CTCGATT96700.011.9191221
CTCGTAT103550.011.7188571
CTCAAAT393700.011.0675921
CGAATGT119900.010.9805263
CTCGGTT170750.010.5367361
GTCGTTT138750.010.1303361
CTCGGAT248850.010.1293181
TCGTTTT222800.010.0308852
CGCGTTT27200.09.8184191
CCGGGAT127550.09.6607511
CTCAGAT347650.09.6495341
CCGGTTT118250.09.5092371
TCGGTTT142100.09.4958632
ACGATAC55500.09.47331960-61
CTCAATT252800.09.4150421
Produced by FastQC (version 0.11.5)