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4R023-L3-P07-ATGTCA-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L3-P07-ATGTCA-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L3-P07-ATGTCA-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34673122
Sequences flagged as poor quality0
Sequence length60-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG64950.013.33751344-45
CCCGTAT42100.011.7927231
TCGACCG42850.011.69289730-31
CTCCGAT105350.010.7145221
CGATCCG48150.010.52415582-83
GTCCGTT58900.010.1785281
CCCGAAT74800.010.1438711
CTCGTTT121850.010.0708481
CCGGGAT98350.09.8579431
CCCGTTT98000.09.8453571
CTCCGTT116200.09.6334551
CCGGTAT49750.09.6027891
CGGGTAT45150.09.44004251
CCCGATT49950.09.3768031
CTCGAAT124200.09.276921
CCCGGTT99650.09.2123211
GTCGGTT65400.09.1669011
TATGCCG47900.09.04317148-49
GTTCGAC57000.08.99577828-29
CCCGGAT134400.08.9562071
Produced by FastQC (version 0.11.5)