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4R023-L3-P08-GTGAAA-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L3-P08-GTGAAA-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L3-P08-GTGAAA-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42923514
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT155300.013.0610691
CGCGTAA8350.012.9033841
CTCCGAT142150.012.1272761
CTCGTTT157800.011.3995191
GTCGGTT82700.011.1022981
CTCAAAT322750.011.074411
TCGAATG83150.010.5351722
CTCGTAT92350.010.449431
CCCGAAT92800.010.398761
CTCCGTT139150.09.9311871
CTCGATT79050.09.8963931
CTCGGAT216250.09.8563831
CCGGGAT116350.09.5823691
CTCGCAT95100.09.5069041
CTCGGTT147200.09.3880751
CGAATGT94850.09.3837963
CTCAGTT315300.09.2114931
CGCGTTT22900.09.2053291
GTCGTTT113950.09.12643051
TCGTTTT182500.09.0866272
Produced by FastQC (version 0.11.5)