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4R023-L4-P1-CGATGT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L4-P1-CGATGT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L4-P1-CGATGT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46073440
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT177650.015.2718721
CGCGTAA12000.014.0572421
CTCGTTT173300.012.6269141
TCGAATG101400.012.4768432
CGAATGT110150.011.4857193
CTCCGAT149000.011.4470621
CTCAAAT356650.011.2725961
CCCGAAT97000.010.67577651
CTCGATT81450.010.5853611
CTCGGAT221100.010.5328661
CTCAGAT309800.010.1035481
CTCGTAT97950.010.0460141
CCGGTTT106550.09.9827761
GTCGGTT90700.09.8674511
ACGATAC49550.09.66183760-61
CTCAATT227750.09.6286711
CTCATTT421550.09.5926931
CGCTATT28750.09.4529871
CTCGTCT212350.09.3560491
GTCGTTT121450.09.2210261
Produced by FastQC (version 0.11.5)