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4R023-L4-P4-GCCAAT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L4-P4-GCCAAT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L4-P4-GCCAAT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47257193
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT180400.011.3784981
CTCGTTT174050.011.2012541
CTCCGAT150900.011.118371
CTCGATT82500.010.8499271
CTCAAAT349900.010.3801861
TCGAATG96350.010.3117292
CGCTAAT24150.010.0909851
CCGGGAT123350.010.0682521
CTCGGAT232400.09.8811461
CGATTAG80550.09.8324684
CTCGTAT111200.09.6089821
CGAATGT106000.09.5498223
CCCGAAT102300.09.4371081
CGCTATT26200.09.3014241
GTCGGTT95200.09.2055981
CTCGGTT155750.09.1172091
CCGGTAT64650.09.0612651
CGCGTTT27600.08.9994121
CGCGATA23850.08.98922594
GTCGTTT129850.08.98679351
Produced by FastQC (version 0.11.5)