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4R023-L4-P6-GATCAG-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L4-P6-GATCAG-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L4-P6-GATCAG-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35168405
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAT114950.012.83921
CTCGAAT134750.012.7259061
TCGAATG73650.011.578042
GTCGGTT67750.011.4106671
CTCAAAT275900.011.3948141
CTCGTTT130900.011.2031741
CTCGTAT83950.010.603991
CGCGTAA5557.944031E-610.1303561
CGAATGT83750.010.0698763
CTCGATT62050.09.9670931
CTCAGAT239150.09.7565291
CTCGTCT165550.09.6790641
CGATTAG60650.09.501774
CCCGAAT73650.09.4787251
CTCGGTT115250.09.4722121
CTGGAAT362050.09.446931
CGCATAT27900.09.4041641
GTCGTTT93200.09.3504711
CCGGTTT79800.09.335351
CTCATTT317450.09.3277771
Produced by FastQC (version 0.11.5)