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4R023-L4-P7-ATGTCA-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L4-P7-ATGTCA-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L4-P7-ATGTCA-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39940958
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT156150.012.9344221
CTCGTTT150550.012.4817471
TCGAATG81050.012.0838412
CTCAAAT297550.011.8274661
CTCCGAT129850.011.7287871
GTCGGTT81450.011.1614951
CTCGTAT85750.010.8750361
CTCGATT71550.010.4790541
CTCGCGT18100.010.3560271
CGAATGT90550.010.2468053
CCCGAAT85350.09.937721
CGATTAG66900.09.8764384
CTCAGAT271650.09.7982921
CGCGTAA6707.0193164E-79.7918551
TCGTTTT167500.09.5960182
CTGGAAT399400.09.3980161
GTCGTTT107800.09.3461221
CTCGGAT189750.09.3104631
CCGGGAT103850.09.2954941
CGCATAT30350.09.2641231
Produced by FastQC (version 0.11.5)