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4R023-L4-P9-ACTGAT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L4-P9-ACTGAT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L4-P9-ACTGAT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57191356
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT224400.014.4721361
TCGAATG116150.013.6773732
CTCGTTT209550.012.2326871
CGAATGT128100.011.8527223
CTCGATT103350.011.6531921
CTCGTAT130850.011.4245521
CTCCGAT189750.011.0888581
CTCAAAT440550.011.0839551
GTCGGTT110600.010.8045641
CTCGGTT189300.010.4963291
CTCGGAT268500.010.4894431
CGCTTAT69200.010.4288611
CGCGATT18150.010.3277461
CGCTATT31700.010.2002791
CTCATTT534900.010.0137291
CCCGAAT118250.09.98669051
CGCTAAT32600.09.7749271
CTCATAT252150.09.571291
CTCAATT283200.09.564381
CTCAGTT425950.09.549561
Produced by FastQC (version 0.11.5)