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4R019-L5-P1-ATCACG-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Fri 14 Apr 2017
4R019-L5-P1-ATCACG-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R019-L5-P1-ATCACG-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49347134
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT195600.012.9663141
TCGAATG103600.011.4725882
CTCCGAT150650.011.461551
CTCGTTT179500.010.7403621
CGCTATT31800.010.59476951
CGAATGT115150.010.1182163
CTCAAAT372450.09.9253371
GTCGGTT109950.09.7885581
CCGCTAT40700.09.65763951
GTCGAAT76250.09.5121341
CTCATAT193100.09.3296341
CCCGAAT100100.09.2091231
CGTCTGA1005900.09.05071294
CGATTAG79700.09.0412654
GTCGTTT147250.09.0250411
CCGGCAT130150.08.9524371
TCGTTTT229250.08.9403032
CCGGGAT125250.08.9290711
CTCGGAT246950.08.7732021
CCGGGTT149300.08.6817261
Produced by FastQC (version 0.11.5)