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4R019-L5-P2-CGATGT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R019-L5-P2-CGATGT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R019-L5-P2-CGATGT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73523737
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT311350.013.9024121
TCGAATG161200.012.9759942
CCGCTAT55100.012.0596311
CTCGTTT285550.011.7662911
CTCCGAT229850.011.462021
CGAATGT184600.011.3054243
CTCAAAT583000.010.8679451
TCGTTTT354300.010.1699252
GTCGTTT219450.010.1287371
CTCATAT303550.010.0048761
CGCTAAT60300.09.9332171
CCGGCAT195200.09.924711
ACGATAC89000.09.66940860-61
CTCGGAT367650.09.6224251
CGATTAG123200.09.6092864
CCCGAAT151500.09.5751671
CTCGATT116500.09.4794551
CGCGTTT39600.09.3353191
CTCGTAT178050.09.2249161
CCGGGAT184300.09.2167661
Produced by FastQC (version 0.11.5)