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4R019-L5-P3-ACAGTG-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R019-L5-P3-ACAGTG-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R019-L5-P3-ACAGTG-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58410181
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG96350.018.71543944-45
TCGTATG129700.017.88829842-43
CTCGTTT225950.013.5191391
CTCGAAT243900.013.2721861
CGTATGC141900.012.87259242-43
TCGAATG118400.012.7614642
CTCCGAT183300.012.5049441
ATGCCGT198800.011.9412146-47
CTCAAAT454300.011.8002191
CGAATGT143900.011.05227853
GCCGTCT218500.010.8646348-49
GTCGGTT124350.010.7589141
CCGCTAT41000.010.7248741
GTATGCC222350.010.63436144-45
CCCGAAT120300.010.6156161
TCGTTTT271150.010.6099952
CTCATAT245800.010.5432881
CGATTAG96150.010.5084194
GTCGTTT169550.010.42898751
CTCGGTT191300.010.4170041
Produced by FastQC (version 0.11.5)