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4R019-L5-P4-GCCAAT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R019-L5-P4-GCCAAT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R019-L5-P4-GCCAAT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64706173
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG144850.016.474192
CTCGAAT276800.015.3486841
CGAATGT165650.013.6140653
CTCGTTT250800.012.4996731
ACGATAC79050.012.25035960-61
CTCAAAT503950.011.6800461
CGCGTAA19300.011.3944121
CGCGTTT36200.010.59881
CTCCGAT206150.010.5313981
CTCGTAT168250.010.3173991
CGGGAAT115400.010.2986271
CTCGATT102400.010.2809671
CCGCTAT49850.010.0431521
CGCATAT48050.09.9324911
GTCGGTT143800.09.8915891
CTCGGAT317400.09.83264351
CTCATTT583750.09.8108331
CATACGA96850.09.79130556-57
CTCGGTT206000.09.7895211
TCGTTTT305850.09.775632
Produced by FastQC (version 0.11.5)