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4R019-L5-P6-TAGCTT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R019-L5-P6-TAGCTT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R019-L5-P6-TAGCTT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82048416
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT345000.014.5671531
TCGAATG175150.013.8926162
CTCGTTT321200.013.2864451
CTCCGAT256000.012.2468481
CTCGATT130300.012.0665851
CTCAAAT660450.011.9526621
CGAATGT206550.011.666943
GTCGTTT239750.011.18371
TCGTTTT381600.011.17122652
CTCGGAT400350.010.7531941
CTCGTAT219650.010.5028021
CTCGGTT265900.010.4885981
CTCCTAT279850.010.4172291
CTCGTCT387850.010.31553651
CGATTAG140900.010.2285334
CCCGTAT102950.010.090581
CCCGAAT167050.010.0282711
ACGATAC99350.010.00773160-61
CTCATTT750200.010.0049931
CCGCTAT64250.09.977851
Produced by FastQC (version 0.11.5)