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4R024-L6-P9-GTGAAA-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Mon 17 Apr 2017
4R024-L6-P9-GTGAAA-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R024-L6-P9-GTGAAA-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39956929
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG87200.012.3013432
CTCGAAT185950.012.24259951
CGATTAG65150.011.6473054
CTCGTAT94700.011.1292531
CTCCGAT129850.011.0025011
CGAATGT100550.010.9008053
GTCGGTT90350.010.6281881
CTCGTTT152450.010.5697451
CCCGTTT104700.010.5584171
CTCAAAT317050.010.3567561
CGCCTAT26850.010.2929821
TCGGTTT113600.010.267262
CTCGGAT204250.010.2283681
CTCCGTT134450.09.9292761
GTCGTTT124300.09.9110151
CTGGAAT400950.09.4980361
CCCGTAT54700.09.4197391
CCCGAAT81450.09.374111
CCCGGAT142000.09.2693981
TCGTTTT195000.09.2002092
Produced by FastQC (version 0.11.5)