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4R024-L5-P9-ATGTCA-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Mon 17 Apr 2017
4R024-L5-P9-ATGTCA-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R024-L5-P9-ATGTCA-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40702603
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG92050.012.5714292
CTCGAAT189350.012.2228681
CCGCTAT35150.011.8624991
CTCCGAT128300.011.6851641
CTCGTTT159000.011.3736981
CTCGTAT93950.011.3198471
CCCGTTT109050.011.2990411
CCCGAAT87100.010.7040081
CGAATGT108550.010.7035273
CCCGTAT55150.010.5338631
GTCGGTT91050.010.2396381
ACGATAC51300.010.204246560-61
CCCGGAT146150.010.1297751
CTCAAAT324150.010.1172741
TCGTTTT201300.09.7750052
CGATTAG64800.09.7601234
GTCGTTT125100.09.6996031
CGCGTAA8803.612513E-99.5829971
CCCGATT57050.09.4439511
GTTCGAT73550.09.3636711
Produced by FastQC (version 0.11.5)