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4R023-L2-P02-CGATGT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Fri 14 Apr 2017
4R023-L2-P02-CGATGT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P02-CGATGT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48968831
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG104850.014.7435082
CTCGAAT219000.013.6468331
CTCGTTT192400.012.3440661
CGAATGT123300.012.2714693
CTCGTAT114900.012.1493091
CTCCGAT153100.011.9022681
CTCAAAT394450.011.7451811
ACGATAC59100.011.55542160-61
CCGCTAT36400.011.0675741
GTCGGTT112600.010.8581911
CCCGTTT124850.010.8433781
GTTCGAT84000.010.59571
GTCGTTT146300.010.4382721
TCGTTTT241400.010.0130822
TCGGTTT141450.010.0013662
CGATTAG77350.09.9319494
CCCGAAT102800.09.8427391
CTCGATT79300.09.68781951
CGCGTAA8853.9908628E-99.5276221
CTCATTT444700.09.4067521
Produced by FastQC (version 0.11.5)