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4R023-L2-P03-ACAGTG-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Fri 14 Apr 2017
4R023-L2-P03-ACAGTG-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P03-ACAGTG-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40144141
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG90000.016.3412062
TATGCCG47500.015.97122744-45
CTCGAAT187050.014.6986471
CGAATGT104850.012.9547233
CTCGTTT157700.012.9494631
CTCCGAT124100.012.7945461
TCGTATG72600.012.44893442-43
CTCGATT57700.012.2573921
CTCAAAT325150.012.1290081
ACGATAC54300.011.23626460-61
CGATTAG67750.011.1303044
CTCGTCT196400.010.5409011
CTCGGAT204900.010.3322151
GTTCGAT74100.010.2398541
CGCGTAA11450.010.2266041
GTCGTTT124700.010.2164361
CTCATTT360700.010.1025361
CTCAGTT297700.010.1007391
CTTTATT377700.010.0570551
TCGTTTT199350.09.9620012
Produced by FastQC (version 0.11.5)