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4R023-L2-P11-TTAGGC-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L2-P11-TTAGGC-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P11-TTAGGC-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39404276
Sequences flagged as poor quality0
Sequence length60-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT163000.013.79299451
TCGAATG88550.013.1180132
CGCGTAA28150.012.812011
CGCTAAT38600.012.37706851
CGAATGT94300.012.2685423
CTCAAAT313200.012.1882341
CTCCGAT113800.011.8537331
CTCGTTT142200.011.5614541
CGCATAT27850.011.1000181
GTCGGTT82900.010.6785481
CTCGTAT87450.010.6585521
ACGATAC47150.010.35213260-61
GTCGTTT117250.010.2665661
CTCGATT57800.010.2105151
TCGACCG43600.010.1486926-27
CGATAAC43750.09.9565721
CGCGGAT31100.09.9400471
CTCATTT351700.09.9084451
CGATCCG45650.09.83939378-79
CTCATAT175200.09.8382681
Produced by FastQC (version 0.11.5)