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4R023-L2-P07-ATGTCA-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L2-P07-ATGTCA-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P07-ATGTCA-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45880839
Sequences flagged as poor quality0
Sequence length60-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG82350.014.156424544-45
TATGCCG62800.012.82258246-47
CTCGAAT193050.011.6674181
CCCGAAT101550.011.6203751
CCCGTTT120450.011.1576981
CTCCGAT145700.011.0881481
GTCGTTT139350.010.686111
CCCGTAT62450.010.1977871
CGCATAT29950.010.006511
CCCGATT65100.09.9984641
CGCCTAT28250.09.9456291
CCGCTAT39300.09.8897431
TCGAATG93650.09.8004892
CTCAAAT323050.09.7119071
TCGTTTT212050.09.6945082
CTCGTTT170100.09.6903141
CGCGTAA8702.9922376E-99.6884151
CGATTAG70100.09.6859964
GTCGGTT106750.09.4751341
CCGGGAT111000.09.3654681
Produced by FastQC (version 0.11.5)