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4R023-L2-P08-GTGAAA-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L2-P08-GTGAAA-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P08-GTGAAA-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36636332
Sequences flagged as poor quality0
Sequence length60-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT156650.015.6975251
TCGAATG81500.015.3446132
CGAATGT95150.013.6356063
CTCGTTT137550.012.32679751
CTCCGAT105250.012.2825711
ACGATAC45650.012.08006260-61
CTCAAAT292750.011.91961
CGCATAT27500.011.4115431
CTCGATT50100.011.3122741
CGCGTAA20750.011.2863671
CGCTATT28350.010.9041831
CTCGGAT164650.010.6108311
TCGTTTT167850.010.5759682
CTCGTAT82200.010.5414941
GTCGTTT107400.010.5102991
CTCATTT334550.010.2343111
CTCGTCT164650.010.1841221
CTCAGAT268550.09.906621
GTTCGAT72150.09.7377191
CAACGAT67150.09.6955191
Produced by FastQC (version 0.11.5)