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4R023-L2-P10-ACTGAT-Sequences.txt.gz FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L2-P10-ACTGAT-Sequences.txt.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P10-ACTGAT-Sequences.txt.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44989398
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG102800.019.0198142
CTCGAAT204900.015.9146931
CGAATGT128350.015.7830283
ACGATAC64300.013.48492360-61
CTCGTTT180650.012.7434591
CGCGTAA10850.012.686791
CTCAAAT352200.012.3719121
GTCGGTT99900.011.9734311
CTCCGAT148800.011.9622211
CGATTAG79150.011.2825634
GTTCGAT85000.011.1684931
CTCGATT71900.011.1568431
CATACGA77950.010.79141756-57
TCGGTTT122600.010.6576232
CGATACT80400.010.57999860-61
CCGCTAT38200.010.4375711
CGCATAT29800.010.3533431
TCGTTTT222350.010.2520482
GCATACG85600.010.15639456-57
TACGATA81950.010.12175758-59
Produced by FastQC (version 0.11.5)