SCN Data - Analysis Results
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This section provides the identifiers and notations used to report the analysis results on this website.
It also provides a description of some parts of the analysis and some comments that may help understand the results.

Analysis Procedure

  • RNA Seq Analysis
    Read trimming was done using Trimmomatic. The first 5 base pairs on both reads were cropped.
    Read mapping, transcript and isoform detection,and differential expression were done using the Tuxedo Suite (Tophat and Cufflinks)
    Tuxedo
  • Detecting Oscillation
    Detecting oscillating transcripts (P-Value, Q-Value, Period, Amplitude, Lag) was done via:
    BioCycle
  • Functional Analysis
    Functional Analysis was performed using GO Terms
    Please click on links under tab for detailed reports of GO Terms for oscillating transcripts.
  • TFBS Enrichment
    TFBS Enrichment was performed using MotifMap and CHiPSeq experiments obtained from ENCODE
    Please use table to see specific experiment identifiers.
  • Visualization
    Please visit Circadiomics for transcript visualization.
    Select Transcriptome
    Datasets:
    MOUSE_SCN_SALINE (Biocycle)
    MOUSE_SCN_DZnep (Biocycle)

Venn Diagrams

Circadian Transcripts


Phase Analysis

Saline Alone


DZnep Alone


Oscillating in both condition in Saline


Oscillating in both condition in DZnep


Heatmaps

Circadian in Saline-Alone

Circadian in DZnep-Alone

Circadian in BOTH Conditions


Data Download

Data Visualization

KO CT12 DZNep

KO CT24 DZNep

WT CT12 DMSO

WT CT24 DMSO