SCN Data - Analysis Results
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This section provides the identifiers and notations used to report the analysis results on this website.
It also provides a description of some parts of the analysis and some comments that may help understand the results.

Analysis Procedure

  • RNA Seq Analysis
    Read trimming was done using Trimmomatic. The first 5 base pairs on both reads were cropped.
    Read mapping, transcript and isoform detection,and differential expression were done using the Tuxedo Suite (Tophat and Cufflinks)
    Tuxedo
  • Detecting Oscillation
    Detecting oscillating transcripts (P-Value, Q-Value, Period, Amplitude, Lag) was done via:
    BioCycle
  • Functional Analysis
    Functional Analysis was performed using GO Terms
    Please click on links under tab for detailed reports of GO Terms for oscillating transcripts.
  • TFBS Enrichment
    TFBS Enrichment was performed using MotifMap and CHiPSeq experiments obtained from ENCODE
    Please use table to see specific experiment identifiers.
  • Visualization
    Please visit Circadiomics for transcript visualization.
    Select Transcriptome
    Datasets:
    MOUSE_SCN_SALINE (Biocycle)
    MOUSE_SCN_DZnep (Biocycle)

Venn Diagrams

Circadian Transcripts


Phase Analysis

Saline Alone


DZnep Alone


Oscillating in both condition in Saline


Oscillating in both condition in DZnep


Heatmaps

Circadian in Saline-Alone

Circadian in DZnep-Alone

Circadian in BOTH Conditions


Data Download

Data Visualization

Pathway Enrichment

Data Download

Pathway Enrichment

MotifMap TFBS, CHiPSeq, and RBP Enrichment

Data Download

MotifMap TFBS, CHiPSeq, and RBP Enrichment

Data Download

In Phase Upstream TFs


KO CT12 DZNep

KO CT24 DZNep

WT CT12 DMSO

WT CT24 DMSO