Worg
33730_GATCAG_L006_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 22 May 2017
33730_GATCAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33730_GATCAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG1107.281791E-414.63511857
TACGTAC950.00465007614.52580634
CCCGTAT1851.7641902E-512.45192051
GTATGCG1700.00153247510.822616
CCGGGAT7050.010.7828331
GCGCTTA1500.007463241510.73309128
CCGGAAT4302.7284841E-1110.7144441
CGCGGAT3451.2281453E-710.0156761
CGCGAGT2352.0115859E-49.8025761
CTCGCGT2352.0115859E-49.8025761
CCCGAAC3358.842417E-79.6270071
GCCGAAT3855.9006197E-89.5734251
CGAGAAT3907.19956E-89.4506881
CTACGTA1950.0047798489.43533133
CGAATAT2200.00121764669.4082913
CCCGGTT4507.0776878E-99.2144211
CGGATCT8100.09.0856473
CGCCACT5604.5474735E-119.0498771
CCGAATA2050.0072131618.9748462
CTCGGTT2851.4087364E-48.89110851
Produced by FastQC (version 0.11.5)