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33730_GATCAG_L006_R1_002.fastq.gz FastQC Report
FastQCFastQC Report
Mon 22 May 2017
33730_GATCAG_L006_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33730_GATCAG_L006_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT2704.5187335E-811.9458371
TCGTATG1550.00952005910.3864943
GATCGCG1550.00952094110.3863615
CCGCAAT2901.458844E-610.3275581
TATACGG1850.00309289889.94573835
CGCTTAT2159.861465E-49.62770629
GTTCGAT2402.4836612E-49.5993331
AATCGGG2656.48605E-59.5464935
TCGGGTC5707.2759576E-129.2800452
ATAGCCG2250.00149365769.20003821
GCGCTTA2000.0058858129.19980728
CGTATGC2000.00588762769.19946344
ATCGAAG4558.796633E-99.0982566
CGGTTAG2300.00182594248.9993623
CGCGGGT3103.587655E-58.918091
CTCGGTT4202.2421591E-78.7765321
CCCGATT2901.6996193E-48.7387031
AAATCGA3958.9481546E-78.7335584
CCCGTAT2400.0026451788.63941
CGAAGAT6204.1836756E-118.5465031
Produced by FastQC (version 0.11.5)