33730_GATCAG_L006_R1_003.fastq.gz FastQC Report
FastQCFastQC Report
Mon 22 May 2017
33730_GATCAG_L006_R1_003.fastq.gz

[OK]Basic Statistics

MeasureValue
Filename33730_GATCAG_L006_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA1050.009024519513.1418369
TCGTATG1606.377854E-512.93681843
CCGGCAT5301.8189894E-129.9998561
TTCGATC2851.3569024E-59.6834582
CTCGAAC5801.8189894E-129.5350951
CGGGAAT4252.7273927E-89.2172581
CGCGTGA2000.00579477759.2172581
CGACAAT3052.9555827E-59.0661561
CGTTAAT2050.00709811928.9924481
CGGACGT2050.0072127268.9749123
CGCGGAT3154.319218E-58.7783411
CCCGTAT2100.0086481968.7783411
CGCAAAC3451.3262334E-58.6829241
AAATCGA3754.175814E-68.58599954
GTCGGAC2708.0434204E-48.5344981
CGCAAAT3256.2298626E-58.5082381
CGCCCAT4903.013338E-88.4648291
CTCGAGT2759.6501113E-48.3793261
CCGCGAT3052.926239E-48.3106431
CGCGGGT3451.2499969E-48.0150071