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33730_GATCAG_L007_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 22 May 2017
33730_GATCAG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33730_GATCAG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAAC2056.372254E-410.0969476
CTCGTAT2352.0166833E-49.8001141
TATGCCG2605.2640262E-59.73017846
CCGGCAT4506.91216E-109.723891
TAGAACG1900.0038575929.68362110
CGCGAGT2159.741543E-49.6405771
TACCGAG3053.0237255E-59.048639
CGACAAT3856.060727E-78.9728321
GATCGAC2100.0087862928.7613725
CTCGGGT7650.08.7304291
CGAAGAT6357.2759576E-128.7043521
TCGGGTA2400.00268694278.6244752
CGGGAAT4903.042078E-88.4600971
ATCTGCG4953.6998244E-88.3631286
CGGGGTT7850.08.2146171
CCGCGAT3103.507829E-48.17202951
CCGGATC6551.4006218E-108.0758442
CGCCCAT5157.0675014E-88.0494141
CTCGGTT2900.0016404087.94147161
CGCCGAC3204.934408E-47.91665361
Produced by FastQC (version 0.11.5)