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33730_GATCAG_L007_R1_002.fastq.gz FastQC Report
FastQCFastQC Report
Mon 22 May 2017
33730_GATCAG_L007_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33730_GATCAG_L007_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGT1101.8318733E-618.81688744
GACGATA1552.885774E-614.8376892
ACCGGTA1107.280893E-414.63535745
CGCGCAT1556.988708E-411.8870911
GTCGTTA1400.004467162311.49920823
CGGCTAA2859.8014425E-811.2974695
TCGGCTA2251.3186855E-410.2215194
CGCGAGT2056.289689E-410.1112761
CGGGGAT5700.09.6973631
CGCGGAT3602.2899985E-79.5963491
CGGCAAT3652.7980786E-79.4648921
CGCAAAT1950.00472432249.4487131
TCGTTAC1950.00478019059.43524924
CTCGGGT6650.09.3510291
CCGGGAT7050.09.1471591
CGGGAAT4558.583811E-99.11125851
CCGCAAT2300.00180236659.0122241
CGATAAC2050.00721219468.9749934
TTACCGG2100.0087868598.76130243
TCGGGTC5004.7421054E-98.7393992
Produced by FastQC (version 0.11.5)