Worg
33730_GATCAG_L007_R1_004.fastq.gz FastQC Report
FastQCFastQC Report
Mon 22 May 2017
33730_GATCAG_L007_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33730_GATCAG_L007_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534198
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGATC1050.00902184813.14169341
CGGGAAT1300.002538721512.4004941
CGGCAAT1505.304836E-412.28239251
CGAGCAT1505.304836E-412.28239251
TCTACCG1505.367835E-412.265187
TCGGGAC1609.229431E-411.4986072
CCGGCAT2654.5827437E-711.2974851
CGGAAAT1650.001180103411.16581251
GCCGAAT1450.005741798311.1176841
GCGCTCT1550.00952000510.38583853
CGTCCCG1850.00309278059.945065532
CGGGCAT3052.7918595E-69.8158471
CGGGGTT2851.3334984E-59.6966271
GAGTCGG2159.863195E-49.626744
CGGGGAT2000.0058196759.2117951
CTCGGGT2000.0058196759.2117951
CCGGCGT2554.573778E-49.0311721
CGAAGAT2554.573778E-49.0311721
CGCCAAC2050.00712855538.9871171
CTTGGAT5654.7239155E-98.1520311
Produced by FastQC (version 0.11.5)