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33730_GATCAG_L008_R1_002.fastq.gz FastQC Report
FastQCFastQC Report
Mon 22 May 2017
33730_GATCAG_L008_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33730_GATCAG_L008_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT3456.91216E-1112.019541
CGACCGT1609.1008213E-411.5187251
GATAACG1954.0192995E-410.6147272
TCGTTAC1850.00309266949.945827521
CGCCGAT2605.1675E-59.74661351
CGCCCAT4506.87578E-109.7269241
GTATAGC2402.5272308E-49.5827396
CCGGGAT7200.09.2789741
AAAATCG3256.3846546E-69.19942953
GGCGAAT2554.5620807E-49.0342941
GATCTAA3154.4185155E-58.7613615
CTCGTCT6601.8189894E-128.7263071
CGCGGTT3205.2106127E-58.6390441
CGCGCTT2759.673796E-48.37725451
CGCCAAT3358.901429E-58.2522211
CGGAGAT6153.1650416E-108.2410381
CCGCAAT3103.4960674E-48.174581
ATCTAGT3703.2721713E-58.0805816
CCGTGAT4852.3084976E-78.0749831
AAAACGC2850.0014006858.0696753
Produced by FastQC (version 0.11.5)