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63246_CGATGT_S1_L001_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63246_CGATGT_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63246_CGATGT_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11719020
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAT15650.010.8820211
CGGAGAT16950.09.6400831
CGACAAT7450.09.5762971
GTCGGTT8750.09.2056021
GAATCGA7500.09.1995934
GTCGCAT6550.09.135331
CGACTAT2801.1747429E-49.0412161
CGGGGAT13800.09.0054791
CGCGGAT7900.08.7394951
CGGCGAT10200.08.5738451
CGGAAAT13100.08.4326121
CTCAGAT47600.08.4126821
CGCGGTT9600.08.3905221
CGACCTA3307.61979E-58.3632654
CGAAGAT19800.08.2524961
CTCGAAT14050.08.1900371
ATATACG4506.585997E-78.1774156
CGCCAAT10150.08.1626021
AATCGAA9350.08.1172875
TTCGATC8250.08.0852842
Produced by FastQC (version 0.11.5)