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63246_CGATGT_S1_L003_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63246_CGATGT_S1_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63246_CGATGT_S1_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11855910
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT7400.011.8180781
CGGGAAT12950.010.6629281
CGGAAAT13400.09.7896011
CGCGGAT8500.09.747171
CCGGGAT17300.09.5781441
CGACGAT4001.0796248E-79.2056611
CTCGGTT10700.09.0335931
ACGCGCG4852.744855E-99.01011220
GTCGATT4101.577082E-78.98113251
GTCGGTT7450.08.9585291
CGAAGAT19050.08.939881
CTGGAAT66450.08.6238131
CTCAGAT47900.08.6002781
CGAGAAT10000.08.5152371
AATCGTA3256.3750726E-58.4918715
ACTCCGC10300.08.4850015
GTCGCAT7650.08.4234811
CTCGGGT18100.08.3919011
CGCGTTT3307.561351E-58.3687831
CGCAATT4701.4186116E-78.3242681
Produced by FastQC (version 0.11.5)