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63247_CAGATC_S2_L001_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63247_CAGATC_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63247_CAGATC_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11760590
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT2003.961459E-511.5064071
GAATCGA8600.010.1625724
CGTATCG4954.0017767E-119.757453
CGCGAAT1900.00383888749.6896061
CGAGAAT11250.09.4096831
CGCGTTT2453.090445E-49.3929841
CCGGGAT16950.09.2322791
GTATCGA5107.1122486E-109.0194154
CCGGAAT13550.08.661651
CGACAAT6651.8189894E-128.6514341
CTCGGTT10400.08.6298061
TACCGGT3205.314446E-58.62488844
GTCGGTT9700.08.5408381
CTGGAAT72650.08.5209181
CGGGAAT13350.08.44665051
GTCGTAT3551.9334482E-58.4272271
GTCGAGT4404.6393507E-78.3682961
CGCAGAT16500.08.3682961
CGCCAAT9350.08.3682961
CGAATCG2759.835908E-48.3635273
Produced by FastQC (version 0.11.5)