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63247_CAGATC_S2_L002_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63247_CAGATC_S2_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63247_CAGATC_S2_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11861399
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA2604.514375E-610.6215581
CCGGAAT13100.010.1891271
CGGGAAT12950.010.1294381
CGCGAAT2352.0324024E-49.7929251
GTCGAAT5451.8189894E-129.7120671
CGAGAAT10700.09.6785221
CTCGCAT12650.09.6419671
CCCGTAT6850.09.4069261
TCGCATT8800.09.1479522
GTCGGTT8250.08.9263991
CTCGAAT16850.08.7409851
CTCGGAT15350.08.6956071
AATCGAA9300.08.6551425
CGCGGAT7500.08.591661
CGAAGAT19650.08.5494981
CTCAGAT48900.08.5182431
CGCGTTT3804.9508526E-68.4786121
CCGGTAT8150.08.4711811
CGCATAT4353.8974213E-78.4646891
CCGACAT11750.08.4219161
Produced by FastQC (version 0.11.5)