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63248_GTGAAA_S3_L001_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63248_GTGAAA_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63248_GTGAAA_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13113625
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT8700.012.1680931
CGAGAAT11900.010.44313051
CCGCAAT9300.010.1457661
CTCGAAT15100.09.6016871
CGCCAAT9450.09.4976631
ATTAGCG3002.4877292E-59.2000916
CGAAGAT20850.09.0508991
CTCGGAT17500.08.9424151
CCCGTAT7800.08.8513721
CCGGAAT15350.08.3958291
CGCTAAT2759.77751E-48.3685691
CGCGTTA2759.833866E-48.36371932
CTCAGAT53150.08.3567611
CTGGAAT71650.08.3510491
CTCGGTT10850.08.2721581
CGGAAAT14200.08.2654361
GTCGGTT10050.08.2436661
CTCGTCT20300.08.049081
CGGAGAT18100.08.0102471
CGTATCG5756.7393557E-98.0002623
Produced by FastQC (version 0.11.5)