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63248_GTGAAA_S3_L002_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63248_GTGAAA_S3_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63248_GTGAAA_S3_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13220926
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT10900.011.6115531
CGACAAC11250.010.8412021
CGAAGAT23500.010.7715681
GTCGGTT10750.010.0610541
CTCGGGT19350.09.7518491
CTCGAAT15250.09.5065911
CTCGCAT13950.09.4027471
CTCGGAT17900.08.9991011
TCGTATG5158.5310603E-108.93290845
CGCGAGT5951.8189894E-118.895391
CGGGGTT22600.08.8585971
TCGGGTC15600.08.8461112
CGACAAT7150.08.6898411
CTCACAT40300.08.6223571
CTCAGAT56500.08.390211
CCCGTAT8800.08.3679951
CTGGAAT73300.08.2566881
CTCCGAT10950.08.196051
CGCCAAT10500.08.1089861
TACCGGT3703.26738E-58.08192844
Produced by FastQC (version 0.11.5)