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63248_GTGAAA_S3_L003_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63248_GTGAAA_S3_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63248_GTGAAA_S3_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13286278
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT9700.012.8111381
CGCGTAA3152.6357156E-911.688941
CGAATCG2603.652076E-711.49937253
GTCGACT6150.011.2256581
CTCGAAT16850.010.6527181
TATGCGC3306.572918E-810.453627
GTCGCAT7200.09.5885831
CGACAAT7850.09.3809331
CGAAGAT21850.09.3735531
CGGGAAT14800.09.32943251
CGGAAAT13850.09.3047331
CTCGGTT11450.09.2452371
CTGGAAT72800.09.23032951
CGACGAT5554.0017767E-119.1221121
CTCGGAT17750.09.0753921
CTCGCAT16350.08.867241
GTATGCG4151.9194886E-78.8666856
CTCGGGT18950.08.8650121
CGGGGAT17650.08.7356611
AACGCGA2901.7313455E-48.72418634
Produced by FastQC (version 0.11.5)