63255_GTCCGC_S10_L001_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63255_GTCCGC_S10_L001_R1_001.fastq.gz

[OK]Basic Statistics

MeasureValue
Filename63255_GTCCGC_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10352658
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1951.3925273E-714.1618121
CGCGGGT8550.010.4971321
CGGAGAT14700.010.1757921
CGGAAAT11800.010.1412971
CTCGGAT16900.09.66815951
GTCGCAT6500.09.5592221
CTCGGTT11700.09.4412081
CTCGTTT11750.09.4010321
CGGGAAT11400.09.0840571
CGACAAT6650.08.9975411
CGGGGAT13850.08.9725561
CCGCAAT7450.08.9580581
CGCAATC5158.5310603E-108.9324042
CGCGGAT6357.2759576E-128.6978051
AATCGAC5055.66979E-98.6531925
CTCACAT28800.08.6298541
CCGGCAT13350.08.6190811
CCGGAAT11800.08.5810981
CTCGGGT16800.08.4928721
CCGGTCT12050.08.4030671