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63256_ACAGTG_S11_L001_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63256_ACAGTG_S11_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63256_ACAGTG_S11_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12691288
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT8900.010.3425621
CGCGTAT2402.5132007E-49.5884161
CTCGCAT12600.09.4970981
CTCGGAT18700.09.4756121
CGGGAAT14050.09.335911
CGGAGAT19350.09.2762361
CTCGTAT8550.09.151051
CGCGGAT9300.08.90794851
CTCGAAT16300.08.8942861
CGGGGAT15150.08.809951
CGGCAAT10550.08.7250051
CGACAAT9250.08.7073171
CTGGAAT76550.08.6577581
GTCGGAT8800.08.6295751
GAATCGA9700.08.5364
CGAAAAT11100.08.5000011
CCGGAAT12800.08.4497921
CGCCAAT11100.08.2926841
CTCGTTT15100.08.2295281
CGAAGAT20700.08.22658351
Produced by FastQC (version 0.11.5)