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63256_ACAGTG_S11_L002_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63256_ACAGTG_S11_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63256_ACAGTG_S11_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12784986
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCAT13150.09.2747931
CTCGGAT20950.09.2267631
CTCGGTT10800.09.162181
CTCGAAC15600.08.8507631
TCAATCG6357.2759576E-128.69240953
CTCAGAT55950.08.6372061
CTGGAAT75450.08.6314011
CGCGGGT12100.08.5581771
CTCGAAT17750.08.4269251
CCGGAAT14800.08.3962651
CGGAGAT19100.08.31323051
CGGGATT12300.08.0448411
CGAAAAC13450.08.0413641
CCGGGAT18500.07.96090271
CGATAAC5807.84712E-97.93516731
AATCCAA50700.07.8475795
CCGGTAT9100.07.83924771
CCGCAAT9400.07.83386751
TCCGATC5003.859259E-77.8200362
AATCGAC5003.8638427E-77.81945475
Produced by FastQC (version 0.11.5)