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63256_ACAGTG_S11_L003_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Tue 21 Mar 2017
63256_ACAGTG_S11_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63256_ACAGTG_S11_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12846536
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA2703.3778633E-912.77827432
CGCGGAT8100.010.5122281
CGCGTAA2707.084165E-610.2281141
ATTAGCG2554.256404E-59.9208356
GAATCGA10750.09.8411314
CGGAGAT19450.09.7022471
CGCGAAC2656.443271E-59.5526711
CTCGAAT16550.09.4555981
GTCCGAT6100.09.4316631
CCGGGAT20500.09.2053021
CGCGAGT5251.2551027E-109.2053011
CGCGTAT2759.6571544E-59.2053011
CTCGAAC15400.08.9662031
CTCGCAT12950.08.8854271
CGAAGAT21050.08.8554571
CTCGGTT11700.08.8512531
GGAATCG14400.08.784383
CGGAAAT14050.08.5173621
GTCGATT6001.9463187E-108.4381941
AATCGAA11500.08.3993785
Produced by FastQC (version 0.11.5)