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63257_GCCAAT_S12_L001_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Tue 21 Mar 2017
63257_GCCAAT_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63257_GCCAAT_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9828238
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT10200.010.8295561
CGGAAAT10000.010.5858911
GTCGGTT7000.010.1913871
AATCGAC4558.185452E-1110.109555
CTCGGGT16450.09.932581
CTCGCAT10450.09.6896031
CGACAAT5950.09.6692481
CCGGAAT10050.09.6172921
CGCAGAT12900.09.2764811
CGGCAAT8250.09.2051231
CCGGGAT14600.09.1420751
CGGGTAT6103.6379788E-129.054221
CGCTAAT2050.00717682768.9806081
CTCAGAT41350.08.9602461
CTCGCGT3359.305189E-68.9303441
CTGGAAT55150.08.8045381
CTCGGAT14000.08.7119921
GATCGAC6103.092282E-118.671845
AATCGGT6900.08.6663745
CGCGGAT7200.08.6298031
Produced by FastQC (version 0.11.5)