63257_GCCAAT_S12_L003_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Tue 21 Mar 2017
63257_GCCAAT_S12_L003_R1_001.fastq.gz

[OK]Basic Statistics

MeasureValue
Filename63257_GCCAAT_S12_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9943336
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT2251.0610154E-511.250551
CGAGAAT9600.010.5473921
CGGAAAT9900.010.4602231
CAATCGA3855.6006684E-910.1552794
CGAAGAT15000.010.1254961
CGCGGAT6500.09.9130731
CCGGGAT15100.09.9060391
CCGGAAT10400.09.7360541
CGGCAAT8800.09.4142011
CGCACAT9650.09.30038451
CGGCAAC9500.08.9627591
CTCGGAT15150.08.961961
AATCGAA8600.08.825095
CTCGATT9650.08.82344151
CTCAGAT39750.08.7418521
CCGACAT11150.08.6683821
CGCCATT11850.08.3505241
CGGGAAT11050.08.3303141
ATCGACG5809.0403773E-108.3271236
TCGTATG3907.0743044E-68.25640843