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63258_ATGTCA_S13_L001_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63258_ATGTCA_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63258_ATGTCA_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8140031
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT4800.011.0265631
CTCGGAT12900.010.3464611
CGCCCAT9100.010.1151461
CGCGGAT4151.8937499E-89.4265851
CGCGCTT3451.3397839E-69.3381861
CGGGAAT7950.09.2626751
CGCCTAT3256.3406587E-69.20478251
GTCGACT4051.3081808E-79.0911441
CTCAGAT35800.09.0633691
CTCGGTT7650.09.0242971
CGCAATT4101.5790647E-78.9802761
CTCGAAT9550.08.67466451
CGAGAAT10350.08.6711721
CTGGAAT45500.08.5978751
TCGCATT7500.08.5872252
CTCAAAT30000.08.4377171
TAAGCGA3002.498575E-48.43341546
CTCGGGT11200.08.424021
CTCGAGT7650.08.4226771
CGGGGAT11250.08.38657951
Produced by FastQC (version 0.11.5)