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63259_CTTGTA_S14_L001_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63259_CTTGTA_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63259_CTTGTA_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8868938
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG2201.0504746E-410.4541387
GTCGATT3753.601599E-910.4316121
CGTATCG3804.5147317E-910.2893053
GCCGTAT4353.783498E-1010.0507411
CTCGACT8050.010.0047431
CCGTATC6750.09.8818222
GCAATCG3551.9074287E-79.7181513
ATTCGAC4507.0940587E-109.7107325
CCGAGAT16450.09.6519921
CTCGCAT8900.09.5663331
CTCGAAT10450.09.4686031
CTCGGTT9000.09.460041
CCGGAAT9100.09.3560841
TCCGATC4202.320121E-89.3098432
CTCGTAT7700.09.2641321
CGGAAAT9550.09.1561741
CTCAGAT38350.09.0603581
AATCGAC3307.750015E-69.0602535
ATCGAAG10950.09.0316116
CCGGGAT13800.09.0042691
Produced by FastQC (version 0.11.5)