Worg
63259_CTTGTA_S14_L002_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63259_CTTGTA_S14_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63259_CTTGTA_S14_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8931744
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT2901.2371856E-711.10936451
CGGAGAT13700.010.7502511
CGCTAAT2201.044411E-410.4601161
TTCGATC6400.010.0626762
CTCGCAT9300.09.8977441
CTCGAAT11250.09.81856251
CGGAAAT10600.09.335161
CCGGAAT8500.09.2049031
CTCGTTT10500.08.5474091
CGCGGAT4852.5920599E-88.5406311
GTCGAAT4051.2715172E-68.5230581
GATCGAC5951.6734703E-108.5035415
CGACAAT5157.9726306E-98.4899591
CGAAGAT15050.08.4097951
CGCATAT3855.905871E-68.36809351
CGAGAAT8300.08.3176821
CGAAAAT8700.08.1997691
CTCAGAT38200.08.1928451
CCGAAAT7950.08.1049451
CTCGGAT12800.08.0902461
Produced by FastQC (version 0.11.5)