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63259_CTTGTA_S14_L003_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63259_CTTGTA_S14_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63259_CTTGTA_S14_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8972422
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT2951.2494638E-811.7007151
CTCGGTT8750.011.5714511
CGCGTAA1500.007435784710.7386571
CGAGAAT8600.010.702981
CTCGAAT10050.010.3036151
CTCGCAT8700.09.7864611
TCGCATT7550.09.7487232
CTCGGAT13700.09.4061231
CCCGTAT4701.542503E-99.3024841
CGGAAAT9900.09.2975391
CTCGGGT12800.09.1686091
CGGAGAT12800.09.1686091
CGGGAAT10150.08.8418221
CGCAGAT10800.08.7358121
CCGGGAT12750.08.6631171
CGAATCG2400.00268722268.6246143
CTCGTTT10900.08.4445531
CGGGGAT12550.08.4344611
CTCGCGT4101.5170135E-68.4188081
CTCAAAT31450.08.4143471
Produced by FastQC (version 0.11.5)