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63263_ACTGAT_S18_L003_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63263_ACTGAT_S18_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63263_ACTGAT_S18_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12946701
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT6900.011.6724651
CGCGATT1700.001525454510.828911
GTCGCAT7850.09.9667351
CTCGGAT18250.09.7089331
CGGAAAT14400.09.4282941
CTCAGAT57900.09.1409831
CGAAGAT21200.09.0091921
CGTATCG6205.456968E-128.9029383
CGACAGT11950.08.8579571
GTCGGTT9900.08.8326711
CTCGAAT15550.08.7310261
TCGCATT12150.08.7080762
CGCAGAT15100.08.6864351
CGGGAAT14900.08.6485921
CTCGGGT20700.08.5598091
CGGCAAT12800.08.449511
CTGGAAT75450.08.4177011
CTTAGAT27050.08.2517891
CGAAAAT13700.08.2303671
AGATCCA56150.08.1508024
Produced by FastQC (version 0.11.5)