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63270_CGATGT_S1_L008_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Tue 21 Mar 2017
63270_CGATGT_S1_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63270_CGATGT_S1_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16889783
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG15100.012.65485743
CGTATGC16400.011.78917444
CGTATCG7650.011.1199613
CTCGGAT25050.011.0254021
TATGCCG18450.010.47231346
CCGGGAT24750.09.7641621
GTATCGA10400.09.7270474
CCCGTAT10250.09.6552931
AATCGGG11950.09.6197515
TATCTCG20200.09.57705739
CTCGTAT20300.09.30112942
GTCGGTT13150.09.2762191
CGGAAAT19650.09.2530611
CTCAGAT70550.09.03657151
CGAAGAT26300.08.5761281
CGCGCAT5157.95626E-98.4911651
CGGGAAT19800.08.4855221
CGGGGAT22000.08.3692831
CTCGGTT17250.08.2722471
CTCGGGT22900.08.2413671
Produced by FastQC (version 0.11.5)