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63282_ATGTCA_S13_L008_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Tue 21 Mar 2017
63282_ATGTCA_S13_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63282_ATGTCA_S13_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8820373
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA2104.9728988E-612.04303554
CGGAGAT12400.010.95099451
CGGAAAT9150.010.8160971
ATTCGCG1550.009544319510.3831456
CGGGAAT10250.010.1044311
CCCGTAT4701.4915713E-109.7938941
CGCGGAT4507.003109E-109.7177171
CGAATCG2851.3623765E-59.6805273
CGCCAAT7550.09.4501331
CGCGAAT1950.0047517299.4423171
CGGGAAC13700.09.4078561
CCGCAAT6750.09.206261
CGCATAT3002.4678478E-59.2062591
CTCGACT8950.09.0005321
ATAGCCG5402.2191671E-108.941092521
CCGAGAT19350.08.9207941
CGCACAT10450.08.8098171
CGGCAAT7900.08.740121
CGTATCG4558.547977E-88.59013753
CGAAGAT14650.08.4835831
Produced by FastQC (version 0.11.5)